Relationship between iodine nutrition level and iodine content in drinking water in Weinan City, Shaanxi Province / 中华地方病学杂志

Objective:To explore the relationship between iodine nutrition level and iodine content in drinking water in Weinan City, Shaanxi Province.Methods:In 2017, in 10 counties (cities, districts) under the jurisdiction of Weinan City, one township (town, sub-district office, hereinafter referred to as the township) was selected in each county (city, district) according to the 5 directions of east, west, south, north, and middle. One primary school was selected in each township, and 42 children aged 8 to 10 (age and gender balanced) in each primary school were selected, urine samples and household edible salt samples were collected to test the contents of urinary iodine and salt iodine, and children's goiter was examined. Twenty-one pregnant women were selected from each township, urine samples and household edible salt samples were collected to test the contents of urinary iodine and salt iodine. At the same time, the drinking water samples of residents in townships were collected to test water iodine content. The correlation between urinary iodine, water iodine and salt iodine was analyzed by multiple linear regression.Results:Urine samples of 2 100 and 1 050 were collected from children and pregnant women, and the median urinary iodine was 254.51 and 172.55 μg/L, respectively. Edible salt samples of 2 100 and 1 050 were collected from children and pregnant women, and the median salt iodine was 24.00 and 24.44 mg/kg, respectively. A total of 232 water samples were collected, and the median water iodine was 26.53 μg/L. A total of 2 100 children's thyroids were examined, of which 65 had goiters, and the goiter rate was 3.10%. The regression model of urinary iodine and water iodine in children was statistically significant ( F = 6.48, P < 0.05), the multiple linear regression equation was = 235.52 + 1.01 x, coefficient of determination ( R2) = 0.119, 11.9% of the change of urinary iodine was related to the change of water iodine. Conclusions:Children's iodine nutrition in Weinan City is at an ultra-adequate level, while pregnant women's iodine nutrition is at an appropriate level. Eleven point nine percent of the changes in children's urinary iodine can be explained by the changes in water iodine.

Application and effect evaluation of stoma skin care powder combined with skin protective membrane in postoperative nursing of anal fistula / 中华护理杂志

Objective To investigate the effect of stoma skin care powde combined with skin protective mem-brane in prevention and treatment of secondary eczema after anal fistula surgery. Methods Eighty-three patients after anal fistula operation were divided into test group and control group. On the basis of routine treatment,the pa-tients in test group were used stoma skin care powder combined with skin protective membrane to protect the peri-anal skin. While the patients in control group were treated routinely. The degree of pruritus,the onset time,the number of skin lesions,the area of eczema and the healing time of eczema were observed in two groups 4 d,14 d and 24 d post-operation. Results Patients in test group had lowerdegree of pruritus,later onset time after dressing, less skin lesions,samller area of eczemaand shorter healing time of eczema than patients in control group in the 3 time nodes (all P<0.05). Conclusion Stoma skin care powder combined with skin protective membrane could effec-tively isolatepostoperative wound secretions and anus polyrrhea,and have preventive and therapeutic effects on sec-ondary perianal eczema after anal fistula surgery.

Diagnosis and surgical treatment for primary presacral tumors / 中华普通外科杂志

Objective To investigate the clinical characteristics and surgical treatment for primary presacral tumors.Methods The clinical data of 42 patients of primary presacral tumors from January 2013 to May 2015 were analysed retrospectively.Results Of the 42 patients,16 cases were asymptomatic while 26 patients had discomfort at the sacral or abdominal region,or difficulty in urinating or defecation.90％ of the cases were digital rectum examination (DRE) positive.Among the 42 patients 36 cases underwent surgical treatment,1 case underwent radiotherapy,5 cases refused surgical treatment.Among those receiving surgical resection,28 cases had trans-abdominal surgery and 4 cases had trans-sacral surgery,while 3 cases had trans-abdominal & trans-sacral surgery,1 case had trans-abdominal and perineal surgery.Tumors were completely resected in 31 cases,and palliatively resected in 5 cases.3 cases suffered from intra-operative presacral hemorrhage.1 case with delayed hemorrhage required surgical intervention.2 cases from incision infection recovered after wound disinfection and dressing.3 cases had postoperative hip or leg numbness;1 case with high fever was cured by intensive antibiotics treatment.Conclusion The low incidence of presacral tumors makes early detection difficult.A diagnosis can be obtained by a positive DRE combined with CT or MRI results.Resection is a therapy of choice after biopsies.

Recent advances in the synthesis of tubulysins.

Tubulysins are a family of natural tetrapeptides in clinical development as a consequence of their potent anticancer activity, even for multi-drug resistant carcinoma. Tubulysins inhibit tubulin polymerization by binding to the peptide binding site located near the vinca alkaloid binding site of tubulin. The limited availability and pharmacological profile of the tubulysins attracted synthetic and medicinal chemists to initiate programs towards their total synthesis. This paper reviews efforts toward the total synthesis of tubulysins and selected structure-activity relationship studies of tubulysin analogs.

Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway

The epithelial-mesenchymal transition (EMT) is involved in neoplastic metastasis, and the RON protein may be involved. In the present study, we determined the role and the mechanisms of action of RON in EMT in Madin-Darby canine kidney (MDCK) cells by Western blot and cell migration analysis. Activation of RON by macrophage stimulating protein (MSP) results in cell migration and initiates changes in the morphology of RON-cDNA-transfected MDCK cells. The absence of E-cadherin, the presence of vimentin and an increase in Snail were observed in RE7 cells, which were derived from MDCK cells transfected with wt-RON, compared with MDCK cells. Stimulation of RE7 cells with MSP resulted in increased migration (about 69 percent of the wounded areas were covered) as well as increased activation of extracellular signal-regulated kinase 1/2 (Erk1/2) and glycogen synthase kinase-3β (GSK-3β; the percent of the activation ratio was 143.6/599.8 percent and 512.4 percent, respectively), which could be inhibited with an individual chemical inhibitor PD98059 (50 μM) specific to MAPK/ERK kinase (the percent inhibition was 98.9 and 81.2 percent, respectively). Thus, the results indicated that RON protein could mediate EMT in MDCK cells via the Erk1/2 pathway. Furthermore, GSK-3β regulates the function of Snail in controlling EMT by this pathway.

Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway.

The epithelial-mesenchymal transition (EMT) is involved in neoplastic metastasis, and the RON protein may be involved. In the present study, we determined the role and the mechanisms of action of RON in EMT in Madin-Darby canine kidney (MDCK) cells by Western blot and cell migration analysis. Activation of RON by macrophage stimulating protein (MSP) results in cell migration and initiates changes in the morphology of RON-cDNA-transfected MDCK cells. The absence of E-cadherin, the presence of vimentin and an increase in Snail were observed in RE7 cells, which were derived from MDCK cells transfected with wt-RON, compared with MDCK cells. Stimulation of RE7 cells with MSP resulted in increased migration (about 69% of the wounded areas were covered) as well as increased activation of extracellular signal-regulated kinase 1/2 (Erk1/2) and glycogen synthase kinase-3ß (GSK-3ß; the percent of the activation ratio was 143.6/599.8% and 512.4%, respectively), which could be inhibited with an individual chemical inhibitor PD98059 (50 µM) specific to MAPK/ERK kinase (the percent inhibition was 98.9 and 81.2%, respectively). Thus, the results indicated that RON protein could mediate EMT in MDCK cells via the Erk1/2 pathway. Furthermore, GSK-3ß regulates the function of Snail in controlling EMT by this pathway.

Action of apoptosis-induced ligand gene in relation to tumor necrosis factor on human colon cancer cell line HT29 / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To evaluate the gene therapeutic efficiency of apoptosis-inducing ligand (TRAIL) related to tumor necrosis factor on human colon cancer cell line HT29.</p><p><b>METHODS</b>Human colon cancer cell line HT29 was transfected with adenovirus-mediated TRAIL gene Ad/GT-TRAIL. The morphological changes, cell growth and apoptosis were measured by phase contrast microscope, MTT method and flow cytometry.</p><p><b>RESULTS</b>Obvious morphological changes in HT29 cells was induced by Ad/GT-TRAIL and Ad/PGK-GV16. The cell suppression percentage and the percentage of apoptotic cells were 54.3% and 11.1%, respectively. When used in combination with Ad/PGK-GV16, HT29 was suppressed to 82.7% and the percentage of apoptotic cells was 24.6%. This result showed significantly enhanced therapeutic efficiency on HT29 and thus inhibiting of its growth (P < 0.05).</p><p><b>CONCLUSION</b>Ad/GT-TRAIL is able to induce apoptosis of HT29 and inhibit its growth. Ad/GT-TRAIL shows significantly enhanced therapeutic efficiency for HT29 when used in combination with Ad/PGK-GV16.</p>

Studies of killing and bystander effect of tumor necrosis factor related apoptosis-inducing ligand gene on hepatic carcinoma cell / 中华消化杂志

Objective To study the bystander effect of tumor necrosis factor related apoptosis inducing ligand(TRAIL) gene and it's mechanism. Methods Full length cDNA of human TRAIL was transfected into SMMC7721 with binary adenoviral vectors system. RT PCR was used to determine the expression of TRAIL gene. By MTT assay the effects of the TRAIL gene on the proliferation of SMMC7721 was studied; The apoptosis inducing ability of TRAIL gene on SMMC7721 was tested by fluorescence activated cell sorting (FACS). Bystander effect by testing the proliferation of the mixed cells of SMMC7721/TRAIL and SMMC7721 with different ratios, and the mechanism of bystander effect by testing the proliferation of SMMC7721 cultured with media of SMMC7721/TRAIL without cell components were also studied. Results TRAIL gene expressed by binary adenoviral vector system was able to inhibit proliferation(91.2%) and induce apoptosis(29.07%) of SMMC7721, significant difference between TRAIL gene and the other three controls were observed(PBS, LacZ, Bax)( P

A Clinic Analysis of 156 Cases of Secondary Barrenness Resulted from Drug Miscarriage before Childbearing / 中国医师杂志

Objective To explore the main reason of secondary barrenness resulted from drug miscarriage before childbearing. Methods Gynecologic examination was performed in 156 patients with secondary barrenness after drug miscarriage. Uterus neck and vaginal smear examination, mycoplasma and chlamydozoon detection, and hysterosalpingograghy were performed in the same time. Results 101 patients (101/156,64.74%) had genital duct inflammation,61 patients (61/156,39.1%) had various degrees of tubal obstruction. Conclusion The main reason of secondary barrenness after drug miscarriage was the tubal obstruction resulted from inflammation, especially chlamydozoon and mycoplasma infection. Drug miscarriage was not so safe before childbearing.